Fig. 4. In vivo hepatic uptake of LDL and HDL cholesterol in mice fed a chow or an FF/HS diet.

Mice fed a chow or an FF/HS diet for 20 days were injected with ³H-cholesteryl oleate-labeled LDL (A) or HDL (C). Uptake of ³H-cholesteryl oleate from LDL (A) and HDL (C) was determined. Values represent the mean ± SE of 10 mice. mRNA expression of Ldlr (B), Scarb1 (D), and Cd36 (E) in the livers was determined by qPCR. Values represent the mean ± SE of 10 mice, relative to mice fed a chow diet, which is defined as 1. Apoe was used as the invariant control. The protein levels of SR-BI and CD36 were determined by immunoblotting. Equal amounts of membrane proteins individually prepared from 6 mice of the same group were pooled and used. Calnexin was used as a loading control (F). **P < 0.01 by Student’s t-test.