Fig. 6. In vivo VLDL secretion from livers of mice fed a chow diet or an FF/HS diet.

(A) Mice were fed a chow or an FF/HS diet for 20 days and were then injected with tyloxapol. Blood was collected at the indicated time points, and concentrations of triglycerides and cholesterol were measured. Values represent the mean ± SE of 8 mice. mRNA expression of genes related to VLDL secretion (B) and triglyceride lipolysis (C) was measured by qPCR. (D) Plasma ApoC-III level was determined by ELISA. Each bar represents the mean ± SE of 10 mice. *P < 0.05, **P < 0.01 by Student’s t-test.