Table 2.
Reduction of US and US-TEON treatment and the synergistic effect (log CFU/mL) against E. coli O157:H7 at different processing intensities and durations.
| Treatments | 64 W/cm2 |
191 W/cm2 |
382 W/cm2 |
573 W/cm2 |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 9 min | 18 min | 27 min | 9 min | 18 min | 27 min | 9 min | 18 min | 27 min | 9 min | 18 min | 27 min | |
| US | 0.76 ± 0.09h | 1.38 ± 0.06f | 1.54 ± 0.11ef | 0.82 ± 0.01h | 1.59 ± 0.10e | 1.66 ± 0.01e | 0.81 ± 0.04h | 1.96 ± 0.05d | 3.13 ± 0.12b | 1.15 ± 0.01g | 2.50 ± 0.03c | 3.52 ± 0.19a |
| US-TEON | 2.16 ± 0.06i | 2.87 ± 0.08h | 4.46 ± 0.11d | 2.93 ± 0.07h | 3.71 ± 0.14e | 4.97 ± 0.11c | 3.37 ± 0.06f | 4.48 ± 0.10d | 6.49 ± 0.14b | 3.63 ± 0.02e | 5.02 ± 0.05c | 7.10 ± 0.02a |
| Synergistic effect | 0.19 ± 0.07f | 0.28 ± 0.10f | 1.71 ± 0.13c | 0.90 ± 0.07e | 0.91 ± 0.13e | 2.10 ± 0.03b | 1.29 ± 0.04d | 1.30 ± 0.03d | 2.15 ± 0.17b | 1.27 ± 0.01d | 1.31 ± 0.04d | 2.37 ± 0.02a |
Note:
The microbial reduction by TEON was 1.21 ± 0.03 log CFU/mL.
Synergistic effect (log CFU/mL) was estimated by applying the following equation: Synergistic effect = RUS-TEON - (RUS + RTEON). Where, RUS-TEON represents the microbial reduction resulting from the application of US-TEON; RUS and RTEON represents the microbial reduction resulting from the exclusive application of US or TEON, respectively.
The experiments were carried out three times. Error bars indicate the standard deviation of three technical replicates.
Values with different letters in the same row showed a significant difference at P < 0.05.