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. 2021 Mar 5;2(1):100371. doi: 10.1016/j.xpro.2021.100371

Figure 3.

Figure 3

Live imaging of cytoskeletal projection dynamics in the germline

(A and B) Stills from Methods videos S1 and S2 in Wilcockson and Ashe (2019) showing a microtubule-rich projection labeled with eGFP.αTub and actin-rich projections labeled with LifeAct.GFP in GSCs (false colored magenta), CBs (green) and 2- or 4-cell cysts (blue). Time is in min:s. Arrowheads indicate the tips of filopodia.

(C and D) Box and whisker plots comparing the lifetime (C) and length (D) of actin projections from the following classes: GSC (magenta), CBs (green), and 2-cell cysts (blue). Dashed lines outline GSC projections. Scale bar, 2 μm (A) or 5 μm (B). See Figure 2 in Wilcockson and Ashe (2019) for further details.