REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Fetal bovine serum	Sigma	F3018
Penicillin-streptomycin	Sigma	P0781
Human insulin	Sigma	I2643
Schneider's Drosophila medium	Thermo Fisher	21720-024
Fibrinogen, bovine plasma	Millipore	341573
Thrombin protease	GE Healthcare Lifesciences	27-0846-01
Yeast	Merck	YSC2
Glucose	Fisher Scientific	10385940
Maize flour	Merck	BCR377
Agar	Melford Biolaboratories	A20020-500.0
Nipagin	Merck	H5501
Propionic acid	Merck	81910
Experimental models: organisms/strains
D. melanogaster; y[1] w[67c23]	Bloomington	RRID:BDSC_6599
D. melanogaster; w[∗]; GAL4::VP16-nos	Bloomington	RRID:BDSC_4937
D. melanogaster; M{w[+mC]=UASp-LifeAct.mGFP6}ZH-2A, w[∗]	Bloomington	RRID:BDSC_58717
D. melanogaster; w[1118]; P{w[+mC]=GAL4::VP16-nos.UTR}CG6325[MVD1], P{w[+mC]=UASp-GFPS65C-alphaTub84B}3	Bloomington	RRID:BDSC_7253
D. melanogaster; w[∗]; P{w[+mC]=His2Av-mRFP1}II.2	Bloomington	RRID:BDSC_23651
Software and algorithms
Fiji	(Schindelin, 2012)	RRID: SCR 002285
GraphPad Prism 7	GraphPad Software	RRID: SCR 002798
Other
PYREX 9 depression glass spot plate	Corning	7220-85
Forceps Watchmaker SS	Scientific Laboratory Supplies	INS4366
FluoroDish cell culture dish 35 mm, 10 mm well	World Precision Instruments	FD3510-100
Leica TCS SP8 AOBS inverted microscope	Leica	NA