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. 2021 Feb 18;24:104–112. doi: 10.1016/j.omtn.2021.02.018

Figure 3.

Figure 3

lnc-CES1-1 did not act in a cis-regulatory manner

(A) ChIP-qPCR analysis of H3K4me1 and H3K27ac enrichment near lnc-CES1-1. (B) The relative expression level of lnc-CES1-1 in the nuclei and cytoplasm. (C) Relative expression level of CES1 after lnc-CES1-1 overexpression. (D) ChIP-qPCR analysis of H3K4me1 and H3K27ac enrichment in HTR-8/SVneo cells. (E) Relative expression level of lnc-CES1-1 after knockdown of STAT4. (F) RNA immunoprecipitation (RIP) followed by quantitative real-time PCR to identify RNA binding proteins. (G) RNA in situ hybridization proximity ligation assay (rISH-PLA) of lnc-CES1-1 and FUS in HTR-8/SVneo cells. ∗p < 0.05. Results are presented as the mean ± SEM.