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| Triton extraction buffer | Final concentration | Amount |
|---|---|---|
| 1% Triton Base Buffer | n/a | 4.425 mL |
| PIC | n/a | ½ tablet |
| 500 mM NaF | 50 mM | 500 μL |
| 200 mM Na3VO4 | 2 mM | 50 μL |
| 0.1 M PMSF | 0.5 mM | 25 μL |
| Total | 5 mL |
For smaller volumes of extraction buffer, you may need to prepare 10× PIC. To do this, dissolve ¼ PIC tablet in 250 μL 1% Triton base buffer. Store 10× PIC-Triton at −20°C.
