2% SDS base buffer	Final concentration	Amount
10% SDS	2%	10 mL
1 M Tris pH 7.4	50 mM	2.5 mL
Milli-Q H2O	n/a	37.5 mL
Total		50 mL

The SDS base buffer can be stored at 20°C–22°C for 2–3 years.