PFA fixative solution | Final concentration | Amount | Final concentration | Amount |
---|---|---|---|---|
10% paraformaldehyde (PFA) | 0.4% | 2 mL | 4% | 4 mL |
10× PBS | 1× | 5 mL | 1× | 1 mL |
Milli-Q H2O | n/a | 43 mL | n/a | 5 mL |
Total | 50 mL | 10 mL |
PFA fixative solution | Final concentration | Amount | Final concentration | Amount |
---|---|---|---|---|
10% paraformaldehyde (PFA) | 0.4% | 2 mL | 4% | 4 mL |
10× PBS | 1× | 5 mL | 1× | 1 mL |
Milli-Q H2O | n/a | 43 mL | n/a | 5 mL |
Total | 50 mL | 10 mL |