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. 2021 Mar 5;218(5):e20200817. doi: 10.1084/jem.20200817

Figure 2.

Figure 2.

GM-CSF is crucial for BM recovery. (A) Kaplan–Meier survival curves. WT (blue line, n = 10) or GM-CSF–KO mice (red line, n = 7) were treated with a single dose of 200 mg/kg 5-FU. In the BM rescue group, KO mice were treated with 5-FU, and 2 d later, they were transplanted with 1 × 107 BM cells from WT mice treated with 200 mg/kg 5-FU 2 d prior (green line, n = 6). Data are pooled from two independent experiments. (B and C) WT and GM-CSF–KO mice were treated with 150 mg/kg 5-FU. PB counts and BM cellularity were analyzed on day 8. (B) WBC, hemoglobin (Hb), and platelet (Plt) counts in PB; n = 9 in WT, n = 7 in KO. Data are pooled from two independent experiments. (C) The numbers of total BM cells, HSCs (CD48−CD150+ LSK cells), LSK cells, CMPs, GMPs, MEPs, and Gr1+ or CD11b+ myeloid cells from femurs and tibias; n = 6–12, pooled from two independent experiments. In the bar charts, the results are shown as mean ± SEM, and each dot represents an individual mouse. Statistical significance was determined by the log-rank test (A) or unpaired Student’s t test (B and C).