Figure 6.

MyD88 deficiency shows a phenocopy of GM-CSF deficiency upon stress by 5-FU. (A) qRT-PCR analyses for the expression of Csf2 in BM ILC2s from WT and MyD88-KO mice; n = 3, representative of two independent experiments. (B) Kaplan–Meier survival curves. WT (blue line) or MyD88-KO mice (red line) were treated with a single dose of 200 mg/kg 5-FU; n = 8 in WT, n = 9 in KO. Data are pooled from two independent experiments. (C) WT and MyD88-KO mice were treated with 150 mg/kg 5-FU, and BM cellularity was analyzed on day 8. The numbers of CMPs, GMPs, and MEPs from femurs and tibias are shown; n = 9 in WT, n = 7 in KO. Data are pooled from two independent experiments. In the bar charts, the results are shown as mean ± SEM, and each dot represents an individual mouse. Statistical significance was determined by ANOVA with the Bonferroni post hoc test (A), log-rank test (B), or unpaired Student’s t test (C). Ctrl, control.