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Experimental design
| Group | Culture method |
|---|---|
| Control | Half medium was refreshed on day 4 and day 7 |
| SR1 | 0.5 μmol/L SR1 was added at the frequency of 12 h−1; half medium was refreshed on day 4 and day 7 |
| Removal of CD34− cells + SR1 | 0.5 μmol/L SR1 was added at the frequency of 12 h−1, CD34− cells were removed with Human Cord Blood Positive Selection Kit II (Stemcell), and isolated CD34+ cells were reseeded with fresh medium on day 4 and day 7 |
