Figure 2.

Nbs1 deletion causes perinatal lethality and depletion of HSCs in mice. A, Kaplan‐Meier survival curve of control (Co) and Nbs1‐HSCΔ (Nbs1Δ) newborns. ‘N’ denotes the number of mice used for the quantification. B, Blood smear (left panels) from P1 control (Co) and Nbs1‐HSCΔ (Nbs1Δ) mice and HE staining of tibia (right panels) from P3 control (Co) and Nbs1‐HSCΔ (Nbs1Δ) mice. C, Representative FACS profiles of haematopoietic stem cells (LSK HSCs) and haematopoietic progenitors (LK progenitors) from control and Nbs1‐HSCΔ mice. Frequencies of LK progenitors and LSK HSCs (in gated bone marrow mononuclear cells) are shown. D, Nbs1 deficiency results in depletion of HSCs (left panel, LSK HSCs) and haematopoietic progenitors (right panel, LK progenitors) in p1 control (Co) and Nbs1‐HSCΔ (Nbs1Δ) mice. Note: N = 3 for each group; ‘P1’ denotes postnatal day 1. Note: *, P < .05; **, P < .01; ***, P < .001. Unpaired Student's t test is used