Figure 5.

Activated DNA damage response in Nbs1‐HSCΔ foetal livers. A, Western blotting analysis on p53 and Chk2 phosphorylation in E15.5 control (Co) and Nbs1‐HSCΔ (Nbs1Δ) foetal liver samples. β‐Actin is used as the loading control. B, qRT‐PCR analysis of p53 and its downstream target genes (p21, Noxa, Puma and Bax) in E17.5 foetal livers from control (Co, N = 5) and Nbs1‐HSCΔ (Nbs1Δ, N = 3) mice embryos. The expression of individual gene in control samples was defined as ‘1’. β‐Actin expression level was used as the internal control. Note: *, P < .05; **, P < .01. Unpaired Student's t test is used