Figure 6.

p53 signaling determines cell fates of Nbs1 deficient foetal liver HSC and LK progenitors. A, The expression of Nbs1, p53 and Bax in control (Co) and Nbs1‐HSCΔ (Nbs1Δ) HSCs and LK progenitors. The expression of these genes in control samples was defined as ‘1’. β‐Actin expression level was used as the internal control. N = 3 for each genotype. B, p21 expression in control (Co) and Nbs1 deficient (Nbs1Δ) HSCs and LK progenitors. The expression of p21 in control samples was defined as ‘1’. β‐Actin expression level was used as the internal control. N = 3 for each genotype. C, Relative expression of p21, p53, Bax in HSCs and LK progenitors from control mouse embryos. The expression of these genes in LK progenitors was defined as ‘1’. β ‐Actin expression level was used as the internal control. N = 3 for each group. Note: *, P < .05; **, P < .01; ***, P < .001. Unpaired Student's t test is used