FIGURE 2.

The expression of Fascin is upregulated in BrCa tissues, and downregulated Fascin restrains cell migration. A and B, In contrast to Fascin expression in paracarcinoma tissues, Fascin was highly expressed in BrCa tissues. Fascin was visualized with strongly positive staining in the cytoplasm. Bar, 500 pixel. Objective lens, magnification, ×20. The boxes were shown the local enlargement tissues. The immunoreactivity score (IRS) was calculated by multiplying the percentage of positive cells by the staining intensity. C and D, MDA‐MD‐231 cells transfected with siRNA targeting Fascin (si‐Fascin) and/or 3HA‐Fascin were allowed to migrate for 12 h. The wound healing area was measured and counted. Bar, 10 μm. Objective lens, magnification, ×20. E and F, MDA‐MD‐231 cells transfected with si‐Fascin and/or 3HA‐Fascin migrated for 6 h in Boyden chambers. The number of migratory cells per field was microscopically counted. Bar, 10 µm. Objective lens, magnification, ×20. *P < .05. **P < .01. ****P < .0001