FIGURE 4.

NLRX1 affects FUNDC1 and NIPSNAP1‐2 protein expression in IEC‐6 cells after HR. A,B, After HR, proteins were isolated and Western blotting was carried out to analyse the levels of NLRX1 before and after reoxygenation. *P < .05 vs 0‐h reoxygenation. C,D, The difference in change of p¹⁸‐FUNDC1 levels after hypoxia and reoxygenation. ^#P < .05 vs control; *P < .05 vs hypoxia. E‐I, The expression of NLRX1, p¹⁸‐FUNDC1, NIPSNAP1 and NIPSNAP2 in HR injury with either empty plasmid or NLRX1 overexpression plasmid. Overexpression of NLRX1 at the reoxygenation phase could reverse FUNDC1 activity. ^#P<.05 vs control group; *P < .05 vs NC_NLRX1+HR group