Table 3. The structure of extruded DMPC/DPPC vesicles.
The optimized parameters describing the lipid bilayer structure, the overall sizes of MLVs and their multilamellar organization are obtained by LIPMIX from the experimental SAXS data of DMPC and DPPC particles in aqueous solutions after 25 passes through the extruder. The error estimates for the model parameters were obtained as a standard deviation of optimized parameters from successful LIPMIX reconstructions.
| DMPC | DPPC | |||||||
|---|---|---|---|---|---|---|---|---|
| Membrane pore diameter | 30 nm | 50 nm | 100 nm | 200 nm | 30 nm | 50 nm | 100 nm | 200 nm |
| Parameters of the lipid bilayer | ||||||||
| z H1 (nm) | 1.86 ± 0.02 | 1.83 ± 0.02 | 1.82 ± 0.02 | 1.84 ± 0.02 | 2.14 ± 0.03 | 2.13 ± 0.03 | 2.12 ± 0.03 | 2.15 ± 0.03 |
| σH1 (nm) | 0.27 ± 0.01 | 0.26 ± 0.01 | 0.25 ± 0.01 | 0.27 ± 0.01 | 0.32 ± 0.01 | 0.33 ± 0.01 | 0.34 ± 0.01 | 0.32 ± 0.01 |
| A 2/A 1 | 1.78 ± 0.04 | 1.78 ± 0.04 | 1.76 ± 0.04 | 1.74 ± 0.04 | 1.78 ± 0.04 | 1.74 ± 0.04 | 1.75 ± 0.04 | 1.76 ± 0.04 |
| z H2 (nm) | 1.71 ± 0.01 | 1.76 ± 0.01 | 1.80 ± 0.01 | 1.78 ± 0.01 | 2.16 ± 0.03 | 2.15 ± 0.03 | 2.14 ± 0.03 | 2.17 ± 0.03 |
| σH2 (nm) | 0.13 ± 0.01 | 0.12 ± 0.01 | 0.12 ± 0.01 | 0.14 ± 0.01 | 0.34 ± 0.02 | 0.33 ± 0.02 | 0.32 ± 0.02 | 0.35 ± 0.02 |
| ρr | 1.53 ± 0.02 | 1.50 ± 0.02 | 1.54 ± 0.02 | 1.53 ± 0.02 | 1.74 ± 0.02 | 1.63 ± 0.02 | 1.65 ± 0.02 | 1.61 ± 0.02 |
| σC (nm) | 0.42 ± 0.03 | 0.40 ± 0.03 | 0.45 ± 0.03 | 0.43 ± 0.03 | 0.42 ± 0.03 | 0.45 ± 0.03 | 0.44 ± 0.03 | 0.43 ± 0.03 |
| Parameters of the MLV size distribution | ||||||||
| R (nm) | 30 ± 1 | 41 ± 1 | 59 ± 1 | 100 ± 2 | 30 ± 1 | 54 ± 1 | 65 ± 1 | 192 ± 3 |
| σ (nm) | 1.2 ± 0.1 | 1.3 ± 0.1 | 4.2 ± 0.2 | 21 ± 1 | 1.2 ± 0.1 | 2.1 ± 0.2 | 9.4 ± 0.5 | 25 ± 1 |
| Structural organization | ||||||||
| Caille parameter η | – | – | – | 0.10 ± 0.01 | – | – | – | – |
| Maximum number of ordered bilayers (SUV/MLV) | SUV | SUV | SUV | SUV + MLV (up to 8 layers) | SUV | SUV | SUV | SUV |