Table 4. The evolution of DMPC vesicles during the extrusion process.

The optimized parameters describing the lipid bilayer structure, the overall sizes of MLVs and their multilamellar organization are obtained by LIPMIX from the experimental SAXS data of DMPC particles in aqueous solutions with different numbers of passes through the extruder. The error estimates for the model parameters were obtained as a standard deviation of optimized parameters from successful LIPMIX reconstructions.

	Membrane pore diameter (30 nm)				Membrane pore diameter (200 nm)
Number of passes	1	5	9	25	1	5	9	25
Parameters of the lipid bilayer
z H1 (nm)	1.89 ± 0.02	1.92 ± 0.02	1.90 ± 0.02	1.86 ± 0.02	1.85 ± 0.02	1.82 ± 0.02	1.82 ± 0.02	1.84 ± 0.02
σH1 (nm)	0.28 ± 0.01	0.27 ± 0.01	0.26 ± 0.01	0.27 ± 0.01	0.29 ± 0.01	0.28 ± 0.01	0.25 ± 0.01	0.27 ± 0.01
A 2/A 1	1.81 ± 0.04	1.82 ± 0.04	1.86 ± 0.04	1.78 ± 0.04	1.73 ± 0.04	1.71 ± 0.04	1.75 ± 0.04	1.74 ± 0.04
z H2 (nm)	1.65 ± 0.01	1.64 ± 0.01	1.65 ± 0.01	1.71 ± 0.01	1.79 ± 0.01	1.80 ± 0.01	1.75 ± 0.01	1.78 ± 0.01
σH2 (nm)	0.17 ± 0.01	0.14 ± 0.01	0.15 ± 0.01	0.13 ± 0.01	0.17 ± 0.01	0.16 ± 0.01	0.15 ± 0.01	0.14 ± 0.01
ρr	1.58 ± 0.02	1.65 ± 0.02	1.64 ± 0.02	1.53 ± 0.02	1.56 ± 0.02	1.56 ± 0.02	1.55 ± 0.02	1.53 ± 0.02
σC (nm)	0.41 ± 0.03	0.44 ± 0.03	0.45 ± 0.03	0.42 ± 0.03	0.42 ± 0.03	0.40 ± 0.03	0.41 ± 0.03	0.43 ± 0.03
Parameters of the MLV size distribution
R (nm)	61 ± 1	37 ± 1	33 ± 1	30 ± 1	96 ± 2	100 ± 2	99 ± 2	100 ± 2
σ (nm)	14 ± 1	3.5 ± 0.2	1.2 ± 0.1	1.2 ± 0.1	14 ± 1	17 ± 1	17 ± 1	21 ± 1
Structural organization
Caille parameter η	–	–	–	–	0.11 ± 0.01	0.12 ± 0.01	0.09 ± 0.01	0.10 ± 0.01
Maximum number of ordered bilayers (SUV/MLV)	SUV	SUV	SUV	SUV	SUV + MLV (up to 8 layers)	SUV + MLV (up to 8 layers)	SUV + MLV (up to 8 layers)	SUV + MLV (up to 8 layers)