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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Cryobiology. 2021 Jan 21;99:78–87. doi: 10.1016/j.cryobiol.2021.01.011

Figure 1.

Figure 1.

Survival and viability of γδ T cells thawed into OpTmizer media. A) Cell counts were performed at 0, 2, 4, 24, and 48 hours post-thaw and the percent recovery was calculated by normalizing counts to the number of cells recovered at 0 hours post-thaw. B) Viability of γδ T cells was measured via Trypan Blue Staining at 0, 2, 4, 24, and 48 hours post-thaw. C) Representative flow cytometry plots visualizing the shift in cell populations up to 24 hours post-thaw by Annexin V and eFluor780 (Live/Dead viability stain) staining. D) Quantification of the percentage of γδ T cells in culture staining positively for Annexin V.