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. Author manuscript; available in PMC: 2021 Mar 9.
Published in final edited form as: Am J Transplant. 2018 Sep 24;19(3):662–673. doi: 10.1111/ajt.15084

FIGURE 3.

FIGURE 3

Mast cell inhibition reduces leukocytic infiltration and suppresses the maturation of antigen-presenting cells in graft. (A) Schematic of experiment design showing the time points of eye drop administration in allotransplant recipients. After 7 days, corneas were harvested to evaluate the frequency of CD45+ leukocytes and mature APCs using flow cytometry. (B) Representative flow cytometric dot plots (left) and cumulative bar chart (right) showing total frequencies of CD45+ inflammatory cells in the corneas of naïve, vehicle-treated, and mast cell inhibitor-treated groups. (C) Representative flow cytometric dot plots (left) and cumulative bar chart (right) showing frequencies of CD11b+MHCII+mature APCs in the corneas of naïve, vehicle-treated, and mast cell inhibitor-treated groups. Representative data from three independent experiments are shown and each experiment consisted of six animals. Data are represented as mean ± SEM (error bar) (t test, *P < .01, ***P < .001)