KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
PEI	Polysciences	Cat#23966-2
Tat peptide (5’-Fluorescein-AAARKKRRQRRRAAA -Lys-TAMRA)	Lifetein	N/A
Critical Commercial Assays
Dual-luciferase reporter assay system	Promega	Cat#E1910
Experimental Models: Cell Lines
HeLa	ATCC	Cat#CCL-2
HEK293T	ATCC	Cat#CCL-11268
Oligonucleotides
The templates for in vitro synthesis (Table S3)	IDT	N/A
The inserts of pFLuc-TAR and mutants (Table S3)	IDT	N/A
The inserts of pFLuc-TAR/RRE and mutants (Table S3)	IDT	N/A
pFLuc-RRE primers for two-step recombinant PCR (Table S3)	IDT	N/A
Recombinant DNA
pcTat	Tiley et al., 1992	N/A
pcRev	Tiley et al., 1992	N/A
pcTat/Rev	Tiley et al., 1992	N/A
pNL4–3	AIDS reagent	Cat #114
pDM128-PL	Fridell et al., 1993	N/A
pFLuc-TAR and mutants	This study	N/A
pFLuc-TAR/RRE and mutants	This study	N/A
pFLuc-RRE and mutants	This study	N/A
Software and Algorithms
NMRpipe	Delaglio et al., 1995	N/A
SPARKY	Goddard and Kneller et al.	N/A
OriginPro	OriginLab	2015
JMP	SAS Institute Inc.	Version 14
Mathematica	Wolfram Research	Version 10.0