Figure 2.

Both loss and gain of RhoB contribute to a decrease in 3D cell motility. A, MDA‐MB‐231 subclones expressing a CRISPR gRNA targeting either a non‐targeting control (NTC), RhoB (RhoB‐1 and RhoB‐2), or containing a vector overexpressing RhoB (RhoB+) were tracked and their trajectories (n = 30–50, N = 2) were plotted using x, y coordinates obtained at 2‐min intervals over a 16‐h time course. Scale bar = 200 μm. B‐E, The (B) average cell velocities, (C) persistence time, (D) total cell diffusivities, and (E) mean squared displacements of the migrating cells were calculated using x, y coordinates obtained from the Metamorph tracking software. Data are shown as mean ± standard error of mean; n = 30–50, N = 2. *, P < 0.05; **, P < 0.01; ***, P < 0.001 vs. NTC (one‐way analysis of variance with Bonferroni posttest for all comparisons)