Figure 4.

Effect of Pluronic F127, curcumin, and PMsCur on the clonogenic ability of MCF‐7 cells. Cells were treated with Pluronic F127 (364 μg/mL), curcumin (5.66 μg/mL), and PMsCur (364 μg/mL) for 24 h. Vehicle control contained 0.02% DMSO, and control represents untreated cells. A, Colony formation assay was performed by crystal violet staining. B, The bar graph represents % of plating efficiency of survived cells. Error bars represent ±SEM of three independent experiments. Significance indicated as ***P ≤ 0.001 between untreated cells and treated cells and ^### P ≤ 0.001 between free curcumin and PMsCur‐treated cells by performing one‐way ANOVA followed by Student‐Newman‐Keuls multiple comparisons test