Figure 3.

Aβ antibody 3D6 modulates Aβ secretion and blocks the synaptotoxicity due to CHO-APP^LDN co-culture. (A) An exemplary immunoblot of CHO cell media collected after 5-day-long treatment with the indicated compounds (DAPT, 18 μg/ml; antibodies, 3 μg/ml) showing APP cleavage products of different molecular weights. (B) Synaptic read-outs in antibody-treated co-cultures at DIV14. Each Homer spot was assigned to the nearest Syp spot within a cut-off distance of 1.0 μm (see Supplementary Fig. 1 for details). Following the assignment of all Homer spots, the fraction of Syp spots assigned by at least one Homer spot and the average number of Homer assignments per Syp spot were calculated. Densities of all Syp and Homer spots detected (white), Syp spots assigned by a Homer spot (grey) and Homer spots assigned to a Syp spot (grey) are also shown. In box plots, red circles, red bars, black squares and red plus signs indicate individual data points, sample median and mean and outliers, respectively. Numbers of microfluidic devices analysed (obtained from at least 3 independent cultures) are given in parentheses. Kruskal–Wallis ANOVA, followed by Wilcoxon rank-sum test. *P < 0.05; **P < 0.01. N/S = not significant.