Table 1.

Oligonucleotides and protocol used for the semi-nested amplification of Plasmodium falciparum Kelch 13 gene

PCR round	Oligonucleotide name	Oligonucleotide sequence (5′ → 3′)	Amplification protocol
			Temperature (°C)	Duration (second)	No. of cycles
First round	K13_PCR_F	CGGAGTGACCAAATCTGGGA	98°C	10 seconds	1
	K13_PCR_R	GGGAATCTGGTGGTAACAGC	98°C	5 seconds	35
			63°C	10 seconds
			72°C	45 seconds
			72°C	2 minutes	1
			20°C	∞	1
Second round (nested 1)	K13_N1_F	GCCAAGCTGCCATTCATTTG	98°C	10 seconds	1
	K13_N1_R	GCCTTGTTGAAAGAAGCAGA	98°C	5 seconds	35
			62°C	10 seconds
			72°C	25 seconds
			72°C	2 minutes	1
			20°C	∞	1
Second round (semi-nested 2)	K13_N2_F	CGCCAGCATTGTTGACTAAT	98°C	10 seconds	1
	K13_PCR_R	GGGAATCTGGTGGTAACAGC	98°C	5 seconds	35
			65°C	10 seconds
			72°C	35 seconds
			72°C	2 minutes	1
			20°C	∞	1