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. 2020 Dec 29;104(3):993–995. doi: 10.4269/ajtmh.20-0206

Figure 1.

Figure 1.

Detection of Plasmodium falciparum SE36 antigen in breast milk of mice and IgG anti-SE36 in mice pup sera. (A) SE36 optical densities (ODs) in breast milk from mothers (dams) that received PBS (open bar) or SE36 (black bar), stomach extracts (PST) of pups breastfed by foster mothers that received PBS (open bar) or SE36 (black bar), positive control SE36 (cross-hatched bar), and negative control PBS (stripped bar). There was a significant difference in SE36 ODs between milk of PBS and antigen-exposed mothers (P = 0.024) but not between the stomach contents of pups breastfed by PBS and antigen-exposed mothers (P = 0.994). Similar results were obtained in three independent antigen recovery experiments. (B) Western blot demonstration of SE36 in breast milk of mice that received SE36 or PBS. The primary antibody was polyclonal mouse anti-SE36, and the secondary antibody was a goat anti-mouse IgG conjugated to horseradish peroxidase; both were diluted 1:1,000. Lane M, molecular weight standard; lanes 1 and 2, milk from PBS-treated mice; lanes 3 and 4, milk from SE36-treated mice after 4 and 6 hours, respectively, after intranasal instillation showing a band of Mr 38,000; lanes 5 and 6, milk from PBS-treated mice; lane 7, PBS-negative control; lane 8, SE36-positive control showing a band which co-migrates slightly below Mr 38,000 probably because of degradation during processing. (C) Results were reported as means ± standard error of the mean (s.e.m) ODs of serum pools from each of three independent experiments and differences between groups assessed using Student’s t test. A value of P < 0.05 was regarded as statistically significant. One representative experiment is presented. IgG ODs in pooled sera from foster mothers that received PBS (open bar) or SE36 (black bar), in pooled sera from pups breastfed by foster mothers that received PBS (open bar) or SE36 (black bar), positive control polyclonal mouse IgG anti-SE36 (cross-hatched bar) and negative control PBS (stripped bar). There was a significant difference in serum IgG anti-SE36 ODs between pups breastfed by PBS and antigen-exposed mothers (P = 0.002) but not between PBS and antigen-exposed mothers themselves (P = 0.787). Keys for (A and C) *P < 0.05, **P < 0.002. NS = nonsignificant. This figure appears in color at www.ajtmh.org.