Table 1.

Sequences of oligonucleotides used in this study for allele-specific PCR

Primers for allele-specific PCR	Sequence (5′-3′)	Product size (bp)
Val 1011
Val1011f	ATTGTATGCTTGTGGGTGACG	–
Iso1011r	GCGGGCTACTTACTACTAGATTTCCAAT	84
Val1011r	GCGGGCAGGGCGGCGGGGGCGGGGCCTACTTACTACTAGATTTCCGAC	104
Met 1011
Met1011f	GTCCTGTATTCCGTTCTTTTT	–
Iso1011r	GCGGGCAGGGCGGCGGGGGCGGGGCCTACTTACTACTAGATTTGCC	62
Met1011r	GCGGGCTACTTACTACTAGATTTACT	82
Ile 1016
Val1016f	GCGGGCAGGGCGGCGGGGGCGGGGCCACAAATTGTTTCCCACCCGCACCGG	102
Ile1016f	GCGGGCACAAATTGTTTCCCACCCGCACTGA	62
Ile1016r	GGATGAACCSAAATTGGACAAAAGC	–
Gly 1016
Gly1016f	ACCGACAAATTGTTTCCC	–
Val1016r	GCGGGCAGCAAGGCTAAGAAAAGGTTAATTA	60
Gly1016r	GCGGGCAGGGCGGCGGGGGCGGGGCCAGCAAGGCTAAGAAAAGGTTAACTC	80