Table 1.
Sequences of oligonucleotides used in this study for allele-specific PCR
| Primers for allele-specific PCR | Sequence (5′-3′) | Product size (bp) |
|---|---|---|
| Val 1011 | ||
| Val1011f | ATTGTATGCTTGTGGGTGACG | – |
| Iso1011r | GCGGGCTACTTACTACTAGATTTCCAAT | 84 |
| Val1011r | GCGGGCAGGGCGGCGGGGGCGGGGCCTACTTACTACTAGATTTCCGAC | 104 |
| Met 1011 | ||
| Met1011f | GTCCTGTATTCCGTTCTTTTT | – |
| Iso1011r | GCGGGCAGGGCGGCGGGGGCGGGGCCTACTTACTACTAGATTTGCC | 62 |
| Met1011r | GCGGGCTACTTACTACTAGATTTACT | 82 |
| Ile 1016 | ||
| Val1016f | GCGGGCAGGGCGGCGGGGGCGGGGCCACAAATTGTTTCCCACCCGCACCGG | 102 |
| Ile1016f | GCGGGCACAAATTGTTTCCCACCCGCACTGA | 62 |
| Ile1016r | GGATGAACCSAAATTGGACAAAAGC | – |
| Gly 1016 | ||
| Gly1016f | ACCGACAAATTGTTTCCC | – |
| Val1016r | GCGGGCAGCAAGGCTAAGAAAAGGTTAATTA | 60 |
| Gly1016r | GCGGGCAGGGCGGCGGGGGCGGGGCCAGCAAGGCTAAGAAAAGGTTAACTC | 80 |