Fig. 3.

CircNEIL3 facilitates the tumorigenesis and metastasis of PDAC cells in vivo. a. Representative images of subcutaneous xenograft tumours (n = 10 for each group). b. Growth curves of tumour volumes, which were measured every five days. c. Tumour weights were analyzed. d. HE and IHC staining of xenograft tumours. The protein levels of Ki67 were analyzed based on IHC staining. The samples were imaged at 400× magnification. Scale bar = 50 μm. e. Representative images and analysis of luminescence intensity in tail vein-injected mouse models (n = 6 for each group). f. Representative images and HE staining of metastatic tumours in the lungs and livers of mice. The HE staining samples were imaged at 100× magnification. Scale bar = 100 μm. g. The number of lung and liver metastatic tumours were determined. All data are presented as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001