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. 2021 Mar 9;13:52. doi: 10.1186/s13148-021-01044-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — MSP and dp_qMSP examples in the analyzed tumor samples for patients 98 and 99. a Example of MGMT promoter methylation in acrylamide gel. The MSP products were loaded and electrophoresed as follows: sample number 98 (lanes 1–4 unmethylated and methylated reactions performed by duplicated), sample number 99 (lanes 5–8, unmethylated and methylated reactions performed by duplicated), lanes 9–10 correspond to unmethylated and methylated reactions using a FFPE negative control sample. Lanes 11 and 12 correspond to unmethylated and methylated reactions using a PBMC control sample. Lane 13 corresponds to PBMC methylated in vitro (IVD) as a positive control, and last line is the water methylation reaction used to discard contamination. b, c. Example of methylated and unmethylated amplification by qMSP. B. Patient number 98, FAM (M) and VIC (U) probes amplified (47.9% methylation). c Patient number 99, only VIC probe amplified (0% methylation). U: Unmethylated. M: methylated. FFPE: Formalin fixed paraffin embedded. PBMCs: Peripheral blood mononuclear cells. NC: negative control. IVD: In vitro Methylated DNA (positive control). NTC (No Template Control)