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. 2021 Mar 8;16:15. doi: 10.1186/s13024-021-00435-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Pyramidal neurons in the hippocampus from AD patients display OPTN in HDA6C-positive aggregates associated with high mitochondrial cholesterol levels. a and b Hippocampal slices from control (CTRL) and AD patients classified into three groups following the “ABC” score: CTRL, intermediate AD (AD III-IV) and high AD (AD VI). Shown are representative confocal photomicrograph of double immunofluorescence for OPTN (green) and HDAC6 (red) at 20X magnification (a) and 63X magnification (b). A negative control slide incubated only with secondary antibodies was included. c Shown are representative confocal photomicrograph of double immunofluorescence for GST-PFO (green) and TOMM20 (red). Sections were incubated with 20 μg/ml GST-PFO for 3 h prior immunolabeling. Insets show a 3-fold magnification of the indicated regions. A negative control without GST-PFO incubation was included. Graphs depict the intensity profiles of GST-PFO and TOMM20 fluorescence in the cross-section. Nuclei were counterstained by DRAQ5 (blue). Scale bar: 50 μm (a) and 25 μm (b and c). In all the cases, the Pearson’s correlation coefficient (PCC) was calculated from 5 individuals per group (at least 6 random fields were analyzed per sample). *P < 0.05; **P < 0.01 (data are mean ± SD)