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. Author manuscript; available in PMC: 2021 Oct 15.
Published in final edited form as: J Immunol. 2020 Sep 14;205(8):2312–2320. doi: 10.4049/jimmunol.2000320

Figure 2. Effects of lubricant in Hb, IgG and IgA detection.

Figure 2.

The same serum sample was measured in triplicate for A) Hb concentration, B) IgG concentration, and C) IgA concentration. All were measured by ELISA. Serum was diluted in different medias, each containing different concentrations of lubricant (10%, 5%, 2.5%, 1.25% or 0% weight/volume). In addition, 1:10 of media from washing 3 lubricated OriCols (not inserted in the rectum) were also compared by ELISA. To have samples within the ELISA ranges, before addition of the lubricant-spiked medias, serum was diluted 1:1,000 for IgA, 1:10,000 for IgG, and 1:1,000 for Hb. There were no significant p values among lubricated OriCols and media containing 0% lubrication. Lines represent medians and bars are the interquartile ranges (IQR) of each sample set.