Figure 4.

In vitro cellular evaluations of drugs and drugMAP gels. A) The summarized drug effects of forskolin (F), Repsox (R), and FR on various cardiac remodeling-associated cells. Sign + represents a positive effect, and sign – represents a negative effect. B) Cumulative drug release profiles from FR/NPs (FR loaded NPs) and FR/drugMAP (F and R loaded drugMAP gel). C) Live and dead staining of neonatal cardiomyocytes cultured in the indicated conditions on day 3, and D) cell viability of neonatal cardiomyocytes. E) Myo-differentiation of neonatal cardiac fibroblasts cultured in the indicated conditions on day 5, and F) mean fluorescent intensity of α-SMA in (E). G) Representative fluorescent images of vascular network formation. Human umbilical vein endothelial cells (HUVECs) are cultured at the indicated conditions for 16 h and stained with Calcein-AM. H) Quantification of junction numbers, tube numbers and mesh numbers. Data are shown as mean ± SD. *p < 0.05 and **p < 0.01 indicate comparisons to blank. ^##p < 0.01 indicates comparisons to R condition. NS represents no significant difference.