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. 2021 Mar 4;16:1819–1836. doi: 10.2147/IJN.S296527

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© 2021 Du et al.

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Epitope prediction and design of the multiepitope peptide rOmp22. In all studies, ***P < 0.001.

Notes: (A) Recombinant plasmid pET-28a-omp22.LaneM is the marker, lane 1 is the pET-28a-omp22 digested with BamHI and Hind III, and lane 2 is the undigested pET-28a-omp22. (B) SDS-PAGE of purified recombinant Omp22 protein. LaneM is the protein marker, lanes 1, 2 and 3 are Bovine Serum Albumin (BSA) calibrators at 1μg, 2μg and 4μg, respectively; lanes 4 and 5 are His-omp22 protein 2μg and 4μg, respectively. (C) Immunization scheme of mice. (D) Indirect ELISA test of candidate B-cell epitopes and Omp22-immunized mouse serum. (E) IFN-γ levels in the splenocytes supernatant. (F) Tertiary structure of the multiepitope peptide rOmp22, three optimal B-cell epitopes and two optimal T-cell epitopes were conjugated in series by 6-aminocaproic acid.

Abbreviation: AHX, 6-aminocaproic acid.