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. 2021 Mar 9;17(3):e1009410. doi: 10.1371/journal.pgen.1009410

Fig 7. TRF1 levels respond to extracellular signals in a PI3K/AKT dependent manner.

Fig 7

A. Representative western blot images of TRF1, SMC1, pAKT(S473) and total AKT (top) and quantification (bottom) of TRF1 protein levels in TRF1+/+, TRF1T358A/T358A and TRF1T273A/T273A cells under normal fed conditions in DMEM+FBS, starved for 24 hours in DMEM without FBS and either refed for 5 hours with FBS or with FBS plus PI3K inhibitor (ETP47037). B. Representative western blot images of TRF1, SMC1, pAKT(S473), total AKT and TIN2 (top) and quantification (bottom) of TRF1 protein levels in TRF1+/+, TRF1T358A/T358A and TRF1T273A/T273A cells starved for 24 hours in DMEM without FBS and either refed for 5 hours with insulin, with insulin plus AKT inhibitor (MK22-06) or with insulin plus PI3K inhibitor (ETP47037). Bars and error bars represent mean values ± SE, n  =  number of independent experiments. Student’s t-test was used for the statistical analysis; *p < 0.05, **p < 0.01, ***p < 0.001 ns, no significant.