Table 1. List of CgYps1 interacting proteins, as identified by immunoprecipitation-mass spectrometry analysis.
| Sl. No | Interactor-encoding ORF (Systematic ID) | S. cerevisiae ortholog | Number of unique peptides (in two replicate samples) | Predicted functions¶ | Protein size (kDa) | Predicted localization# | |
|---|---|---|---|---|---|---|---|
| Oxidoreduction process and energy homeostasis | |||||||
| 1 | CAGL0A00495g | PMA1 | 16 | 2 | Plasma membrane P2-type H+-ATPase; a major regulator of cytoplasmic pH and plasma membrane potential. | 98.3 | Endoplasmic reticulum, Membrane type |
| 2 | CAGL0J00451g | TDH3 | 6 | 5 | Putative glyceraldehyde-3-phosphate dehydrogenase. | 35.9 | Cytoplasm |
| 3 | CAGL0D01298g | TKL1 | 4 | 7 | Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway. | 73.7 | Peroxisome |
| 4 | CAGL0M09581g | ATP1 | 5 | 5 | Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase. | 58.5 | Mitochondrion |
| 5 | CAGL0F01947g | LPD1 | 3 | 5 | Dihydrolipoamide dehydrogenase; the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes. | 53.1 | Mitochondrion |
| 6 | CAGL0F04213g | PET9 | 2 | 5 | Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP. | 32.8 | Mitochondrion, Membrane type |
| 7 | CAGL0H08327g | TPI1 | 4 | 2 | Triose phosphate isomerase, abundant glycolytic enzyme. | 26.9 | Cytoplasm |
| 8 | CAGL0C02343g | ARB1 | 3 | 3 | ATPase of the ATP-binding cassette (ABC) family. | 81.1 | Plastid |
| 9 | CAGL0M13343g | GND1 | 3 | 3 | 6-phosphogluconate dehydrogenase; catalyzes an NADPH regenerating reaction in the pentose phosphate pathway. | 53.5 | Cytoplasm |
| 10 | CAGL0K11858g | PST2 | 2 | 2 | Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress. | 21.0 | Cytoplasm |
| 11 | CAGL0I03916g | ARF2 | 2 | 2 | Putative ADP-ribosylation factor; involved in invasive growth. | 20.6 | Golgi apparatus, Membrane type |
| Ribosome biogenesis | |||||||
| 12 | CAGL0J07238g | RPS12 | 3 | 2 | Protein component of the small (40S) ribosomal subunit. | 15.5 | Cytoplasm |
| 13 | CAGL0K11748g | RPS11A | 2 | 2 | Protein component of the small (40S) ribosomal subunit. | 17.9 | Cytoplasm |
| 14 | CAGL0L04840g | RPS23 | 2 | 2 | Putative ribosomal protein. | 16.0 | Cytoplasm |
| 15 | CAGL0A00979g | RPL38 | 2 | 2 | 60S ribosomal ribosomal protein subunit. | 8.9 | Cytoplasm |
| Other functions | |||||||
| 16 | CAGL0I04356g | TIF1 | 3 | 2 | Translation initiation factor eIF4A. | 44.8 | Nucleus |
| 17 | CAGL0C04389g | HTB2 | 3 | 2 | Histone H2B; core histone protein required for chromatin assembly | 14.0 | Nucleus |
| 18 | CAGL0H02057g | GAR1 | 2 | 3 | Protein component of the H/ACA snoRNP pseudouridylase complex; modify 18S pre-rRNA. | 19.5 | Nucleus |
| 19 | CAGL0D01034g | PSA1 | 2 | 2 | GDP-mannose pyrophosphorylase involved in the synthesis of GDP-mannose for protein glycosylation | 39.4 | Cytoplasm |
¶These functions are predicted based on functions of the corresponding S. cerevisiae orthologs (www.yeastgenome.org).
#Subcellular localization of proteins was determined using DeepLoc server (http://www.cbs.dtu.dk/services/DeepLoc/).