Fig 12. Behavior of motif 2 controller (Fig 2) in comparison with a controller including first-order and second-order autocatalysis in C (Fig 7).

Rate constants for all three controllers: k₂ = 1 × 10⁵, k₃ = 5 × 10⁵, k₄ = 1 × 10⁵, K_M = 1 × 10⁻⁶, K_I = 0.1, n = 4. Additional rate constants for the autocatalytic controllers: k₅ = 10.0, and k₆ = 1.0. Initial concentrations: A₀ = 5.0, E₀ = 0.9, C₀ = 1.0 (when autocatalysis in C is included).