Table 1.
Accuracy of multi-fragment DNA Assembly.
| Construct | Inserts | Fragment Sizes (Total Insert Size) | Vector | colony PCR | Digest | Sequencing |
|---|---|---|---|---|---|---|
| N/A | Hsp16.2-mCherry-Hsp16.2T | 8434, 1900 (1900) | BSP179 | 11/12 | 8/8 | |
| BSP149 | Daf21P, mEGFP, Unc54 T | 8434, 2055, 925, 805 (3784) | BSP179 | 8/8 | 3/3 | |
| BSP147 | Daf21P-mCherry, Unc54 T | 8434, 2055, 901, 805 (3761) | BSP179 | 8/8 | 3/3 | |
| BSP602 | EFT3 P, mScarlet | 9185, 665, 904 (1569) | BSP188 | 6/6 | 2/2 | |
| BSP605 | EFT3 P, mCerulean | 9185, 665, 823 (1488) | BSP188 | 3/3 | 2/2 | |
| BSP606 | EFT3 P, mECitrine | 9185, 665, 823 (1488) | BSP188 | 6/6 | 5/6 | |
| BSP607 | mKO2 | 9794, 713 (713) | BSP605 | 3/3 | 3/3 | |
| BSP608 | mNeptune | 9794, 805 (805) | BSP605 | 3/3 | 3/3 | |
| BSP609 | mTFP | 9794, 767 (764) | BSP605 | 5/6 | ||
| BSP610 | mtagBFP2 | 9794, 784 (784) | BSP605 | 3/3 | ||
| BSP611 | mtagRFP-T | 9794, 805 (805) | BSP605 | 3/3 |