Fig. 3. Growth rate of cells after PLX4032 treatment. TPC1 and 8505C cells cultured in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum. (A) TPC1 cells were treated for 72 hours with 100 nM, 1 µM, and 10 µM of DMSO (CTL) or PLX4032. (B) 8505C cells were treated for 72 hours with 100 nM, 1 µM, 5 µM, and 10 µM of DMSO (CTL) or PLX4032. Relative cell survival rate is shown as percentage of survival vs. control cells after treatment with PLX4032. Values presented here are mean values and standard errors from at least 3 independent experiments. Square box indicates the IC₅₀ value of the TPC1 and 8505C cells. DMSO, dimethyl sulfoxide; CTL, cytotoxic T lymphocyte; IC₅₀, half maximal inhibitory concentration. ^*P < 0.05, ^**P < 0.01 vs. control.