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. 2021 Mar 5;14:711–718. doi: 10.2147/JIR.S299163

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© 2021 Shen et al.

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Pyroptotic cell death indicates by LDH release; cells were pretreated with NAC (10 mM) or PUN (100 μM) +NAC (10 mM), followed by treated with LPS for 5.5 h, ATP for half an hour. The release of LDH was indicates by LDH activity in medium. ^##Indicates P<0.01 vs NC. **Indicates P<0.01 vs LPS/ATP stimulated group. ^$Indicates P<0.05 vs NAC +LPS/ATP group.