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The protein expression levels of NLRP3, caspase‑1, ASC, and GSDMD-N. (A) Representative western-blot images. (B) Quantified histogram of NLRP3, caspase‑1, ASC, and GSDMD-N. Cells were pretreated with NAC (10 mM) or PUN (100 μM) +NAC (10 mM), followed by treated with LPS for 5.5 h, ATP for half an hour. ##Indicates P<0.01 vs NC. *Indicates P<0.05, and **Indicates P<0.01 vs LPS/ATP stimulated group. $Indicates P<0.05, $$Indicates P<0.01 vs NAC+ LPS/ATP stimulated group.
