Fig. 1. Clonal group analyses and characterization of PvRBP2b human mAbs.
a Pie charts representing the repertoire breadth of PvRBP2b161–1454 specific single memory B cell-derived heavy-chain sequences from two Cambodian individuals. The number of variable heavy-chain gene sequences analyzed is indicated in the middle of the pie chart. The colored segments indicate the clonal groups in which at least one human mAb was expressed and their VH genes have been labeled. VH genes for clonal groups that are colored are also shown in Supplementary Data 1. b The frequency of somatic hypermutations (SHM) in IgG heavy-chain variable genes (IgGHV) was determined for the clonal groups of expressed human mAbs from n = 2 individuals. Human mAbs highlighted with a line underneath are from the same clonal group. Each circle represents a clonal member and the line represents the median number of SHM for each clonal group. c Reactivity against PvRBP2b161–1454 using a dilution series of human mAbs (black solid lines). 043038 was used as isotype control (dashed line). Data are presented as mean values of two independent experiments. d Iso-affinity plot showing the range of dissociation rate constants (kd) and association rate constants (ka) of human mAbs (represented as symbols) as measured by bio-layer interferometry. Symbols that fall on the same diagonal dotted lines have the same equilibrium dissociation rate constants (KD) indicated on the top and right sides of the plot. Numeric values for affinity measurements are shown in Supplementary Fig. S2 for each human mAb. e Avidity measurement of PvRBP2b human mAbs using increasing concentrations of ammonium thiocyanate (NH4SCN) by ELISA. Human mAb binding was expressed relative to human mAb binding in the absence of NH4SCN. (i) Strong binding antibodies, (ii) moderate binding, (iii) low binding, (iv) avidity of murine mAbs. Data are presented as mean values of two independent experiments. Source data are provided as a Source Data file.