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. 2021 Mar 9;11:5448. doi: 10.1038/s41598-021-84792-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Diagram illustrating the principle of the HP-LAMP assay. Four steps occur within a single microcentrifuge tube containing the HP-LAMP cocktail. These steps consist of (1) Heat-mediated release of the single-stranded RNA virus (blue) from the virion, (2) Reverse transcription of the single-stranded viral RNA to generate cDNA (purple), (3) Loop-mediated amplification (LAMP) resulting in a decrease in pH, (4) Color change caused by decreased pH. The addition of RNA, DNA and RNAse inhibitors to the HP-LAMP cocktail inhibits degradation of the viral RNA by RNAses naturally found in saliva.