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. 2021 Mar 9;11:5448. doi: 10.1038/s41598-021-84792-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HP-LAMP assay for pooled samples. (A) Illustration of the HP-LAMP assay for sample pooling. A negative matrix was created by pooling 4 known negative saliva samples. A positive or negative pool of 5 samples (N = 5) was created by pooling a known positive or negative sample with the negative matrix. (B) HP-LAMP testing for pooled saliva samples. 20 positive (n = 20) and 20 negative (n = 20) pools were subjected to HP-LAMP assay.