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. 2021 Mar 9;12:1543. doi: 10.1038/s41467-021-21875-0

Fig. 2. Location of the peptide pharmacophore grafting sites.

Fig. 2

The structure of protein scaffold used for lasso-grafting are shown in cartoon representation: a fibronectin 10th type-III domain (Fn10, PDB ID: 1FNF), b carcinoembryonic antigen (CEA, PDB ID: 2QSQ) 1st IgV domain, c signal regulatory protein alpha (SIRPα, PDB ID: 2JJS) 1st IgV domain, d anti-GFP single-domain antibody (VHH, PDB ID: 3OGO), e IgG1 Fc (PDB ID: 1FC1), f human growth hormone (hGH, PDB ID: 1HGU), g serum albumin (HSA, PDB ID: 1E7I), h retinol-binding protein (RBP, PDB ID: 1BRP), i placental alkaline phosphatase (ALP, PDB ID: 1EW2), and j VP3 capsid protein from AAV serotype 2 (PDB ID: 1LP3). In j, structure of the whole virus-like particle comprising 60 VP3 subunits is also shown, with each subunit colored uniquely. For all panels, grafting sites (s1 up to s8) are indicated by spheres with distinct colors as shown in the key. The actual amino acid sequences flanking each grafting site is shown in Table S1. Note that two equivalent sites are present for each site for the homodimers, Fc and ALP.