Figure 5.

Quantification of CZ’s anti-amyloidogenic activity. Determination of the half-maximal disassembling concentrations of CZ particles for insulin (a) and lysozyme (b) and half-maximal inhibiting concentration for insulin (c) and lysozyme (d) quantified using ThT fluorescence assay. ThT fluorescence intensities of amyloid fibrils incubated in the presence of CZ particles (a,b) and amyloid fibrils formed in presence of CZ particles (c,d) were normalized to fluorescence measured after incubation of studied protein amyloid fibrils in the absence of CZs (taken as 100%). Each experiment was performed in triplicates; error bars represent the average deviation for repeated measurements of three separate samples.