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. 2021 Mar 9;11:5496. doi: 10.1038/s41598-021-84185-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of WT, mock and hMT3 in UKF-NB-4 neuroblastoma cells. The cells were transfected with either pcDNA3.1-GFP-TOPO (mock transfection) or pcDNA3.1-GFP-hMT3-TOPO (hMT3). (A) Transfection was validated by confocal microscopy through fluorescence of expressed GFP. The length of scale bar is 10 µm. (B) qRT-PCR showing changes in mRNA encoding hMT3 upon transfection. Data were analysed by comparative CT method and presented as relative fold gene expression (2^–ΔΔCT). (C) Whole-cell lysates immunoblots showing higher expression of hMT3 in UKF-NB-4^hMT3 compared to UKF-NB-4^CDDP cells. GAPDH, loading control. The number below the bands show values obtained by densitometric analysis of bands. (D) Percentages of metabolically active cells after treatment with annotated concentrations of CDDP. **p < 0.001, *p < 0.05. WT, wild-type.