Table 1.
Acceptor substrate specificities of the recombinant BmGalNAcT.
| Substrate, structure | Number of terminal GlcNAc | Number of terminal GlcNAc | |||||||
|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | |||||||
| Specific activity (nmol/h/mg) | Relative activity (%) | Specific activity (nmol/h/mg) | Relative activity (%) | Specific activity (nmol/h/mg) | Relative activity (%) | ||||
| pNP-sugars | |||||||||
| Monosaccharide | Glcβ-pNP | 12.3 ± 2.12 | 0.33 | ||||||
| Galα-pNP | – | – | |||||||
| Galβ-pNP | – | – | |||||||
| GlcNAcβ-pNP | 3.70 ± 0.05 × 103 | 100 | |||||||
| GalNAcβ-pNP | – | – | |||||||
| Disaccharide | Type I | Galβ1,3-GlcNAcβ-pNP | – | – | |||||
| Type II (LacNAc) | Galβ1,4-GlcNAcβ-pNP | – | – | ||||||
| Type III | Galβ1,3-GalNAcβ-pNP | – | – | ||||||
| GalNAcβ1,3-GlcNAcβ-pNP | – | – | |||||||
| (LacdiNAc) | GalNAcβ1,4-GlcNAcβ-pNP | – | – | ||||||
| N-Glycans | |||||||||
| Terminal GlcNAc type |
|
GNM3A | 1 | 15.4 ± 0.50 | 100 | ||||
|
GNM3B | 1 | 7.92 ± 0.33 | 51.4 | |||||
|
GN2M3 | 2 | 16.7 ± 0.17 | 108.2 | 2.1 ± 0.03 | 13.6 | |||
|
GN3M3 | 3 | 21.9 ± 0.20 | 142.2 | 10.1 ± 0.40 | 65.9 | |||
|
GN4M3 | 4 | 23.3 ± 0.47 | 151.4 | 8.3 ± 0.57 | 53.8 | 1.0 ± 0.10 | 6.8 | |
|
GN2M3 + bisect GlcNAc | 3 | – | – | |||||
|
GN3M3 + bisect GlcNAc | 4 | 8.27 ± 0.20 | 53.7 | |||||
| Core Fucose type |
|
GNM3FA | 1 | 10.2 ± 0.07 | 66.2 | ||||
|
GNM3FB | 1 | 9.70 ± 0.27 | 63.0 | |||||
|
GN2M3F | 2 | 11.3 ± 0.07 | 73.5 | 1.4 ± 0.2 | 8.8 | |||
|
GN2M3F + bisect GlcNAc | 3 | – | – | |||||
| Terminal Gal type |
|
GalGN2M3A | 1 | 3.53 ± 0.20 | 22.9 | ||||
|
GalGN2M3B | 1 | 8.29 ± 2.13 | 53.8 | |||||
|
Gal2GN2M3 | 0 | – | – | |||||
|
GalGN2M3FA | 1 | – | – | |||||
|
GalGN2M3FB | 1 | 8.02 ± 1.47 | 52.1 | |||||
| Mannose type |
|
M3 | 0 | – | – | ||||
|
M5 | 0 | – | – | |||||
Relative rates were calculated on the basis of the activity toward GNM3A (100%).