Table 2.

Enzyme properties of recombinant BmGalNAcT expressed in Sf9 cells.

	Optimal condition
Optimum pH	5.5–8.0
pH stability	4.0–7.0
Optimum temperature	20–35 °C
Temperature stability	0–50 °C
Metal-ion dependence : relative activity in the presence of
No addition	2.3 ± 1.4%
10 mM MgCl2	3.9 ± 1.4%
10 mM MnCl2	100%
10 mM CaCl2	12.4 ± 0.7%
10 mM CoCl2	91 ± 0.1%
10 mM ZnCl2	1.2 ± 0.2%

The relative activities in metal-ion dependency were calculated on the basis of the presence of Mn²⁺ (100%).